A novel envelope protein involved in White spot syndrome virus infection
نویسندگان
چکیده
منابع مشابه
PmRab7 is a VP28-binding protein involved in white spot syndrome virus infection in shrimp.
Our aim was to isolate and characterize white spot syndrome virus (WSSV)-binding proteins from shrimp. After a blot of shrimp hemocyte membrane proteins was overlaid with a recombinant WSSV envelope protein (rVP28), the reactive bands on the blot were detected using anti-VP28 antibody. Among three membrane-associated molecules identified by liquid chromatography-tandem mass spectrometry, there ...
متن کاملCharacterization of a novel envelope protein (VP281) of shrimp white spot syndrome virus by mass spectrometry.
The primary structure of a novel envelope protein from shrimp white spot syndrome virus (WSSV) was characterized using a combination of SDS-PAGE and mass spectrometry. The resulting amino acid sequence matched an open reading frame (ORF), ORF1050, of the WSSV genome ORF database. ORF1050 contained 843 nt, encoding 281 aa, and was termed the vp281 gene. Computer-assisted analysis showed that bot...
متن کاملWhite spot syndrome virus VP24 interacts with VP28 and is involved in virus infection.
White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. Herein, the characterization of VP24, a major structural protein of WSSV, is described. When purified virions were subjected to Nonidet P-40 treatment to separate the envelopes from the nucleocapsids, VP24 was found to be present exclusively in the envelope fraction. Triton X-114 extraction ...
متن کاملWhite spot syndrome virus envelope protein VP53A interacts with Penaeus monodon chitin-binding protein (PmCBP).
White spot syndrome virus (WSSV) is the causative agent of a severe disease of cultivated shrimp. Using purified WSSV virions, VP53A encoded by open reading frame wssv067 was identified as a structural protein by SDS-PAGE and proteomics. Immunoelectron microscopy with a gold-labeled secondary antibody revealed that VP53A was distributed on the viral envelope. In order to further explore the lin...
متن کاملIdentification and localization of a prawn white spot syndrome virus gene that encodes an envelope protein.
Among the important challenges to shrimp aquaculture worldwide are the diseases caused by viruses, in particular by white spot syndrome virus (WSSV), which has a genome estimated to contain 305 kb. By analysis and comparison of the WSSV genomic DNA and cDNA libraries, an ORF (vp28 gene) was identified. The gene, encoding a novel 204-amino-acid protein, was expressed in Escherichia coli and puri...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 2005
ISSN: 0022-1317,1465-2099
DOI: 10.1099/vir.0.80923-0